auxiliary reagents

20500 mg/L D-Glucose

0.85% NaCl Dissolvent ; 4 g/L Trypan Blue Solution

1000 mg/L D-Glucose ; 50 mg/L CaCl₂ (anhydrous) ; 100 mg/L MgCl₂·6H₂O ; 36 mg/L Sodium pyruvate

1000 mg/L D-Glucose ; 1000 mg/L NaHCO₃ ; 214 mg/L MgCl₂·6H₂O

10 mg/L Phenol red ; D-Hank's Dissolvent ; 2.5 g/L Trypsin

Ultrapure water Dissolvent

D-Hank's Dissolvent ; 0.2 g/L EDTA·2Na ; 2.5 g/L Trypsin

1000 mg/L D-Glucose ; 350 mg/L NaHCO₃ ; 10 mg/L Phenol red

5 mM Hypoxanthine ; 0.8 mM Thymidine ; 0.85% NaCl Dissolvent

1000 mg/L D-Glucose ; 2200 mg/L NaHCO₃ ; 10 mg/L Phenol red ; 200 mg/L CaCl₂ (anhydrous) ; 200 mg/L MgSO₄·7H₂O

Ultrapure water Dissolvent

Ultrapure water Dissolvent

Ultrapure water Dissolvent

Ultrapure water Dissolvent

Ultrapure water Dissolvent ; 75 g/L NaHCO₃

2000 mg/L D-Glucose ; 2000 mg/L NaHCO₃ ; 200 mg/L CaCl₂ (anhydrous) ; 107 mg/L MgCl₂·6H₂O

5 mM Hypoxanthine ; 0.02 mM Aminopterin ; 0.8 mM Thymidine ; 0.85% NaCl Dissolvent