auxiliary reagents

0.85% NaCl Dissolvent ; 4 g/L Trypan Blue Solution

2000 mg/L D-Glucose ; 200 mg/L NaHCO₃ ; 120 mg/L CaCl₂ (anhydrous)

10 mg/L Phenol red ; D-Hank's Dissolvent ; 0.2 g/L EDTA·2Na ; 0.5 g/L Trypsin

10 mM PBS Dissolvent

Ultrapure water Dissolvent

5 mM Hypoxanthine ; 0.8 mM Thymidine ; 0.85% NaCl Dissolvent

Ultrapure water Dissolvent

10 mg/L Phenol red ; D-Hank's Dissolvent ; 2.5 g/L Trypsin

Ultrapure water Dissolvent ; 75 g/L NaHCO₃

1000 mg/L D-Glucose ; 1000 mg/L NaHCO₃ ; 214 mg/L MgCl₂·6H₂O

1800 mg/L D-Glucose ; 1260 mg/L NaHCO₃ ; 46.8 mg/L MgCl₂

D-Hank's Dissolvent ; 0.2 g/L EDTA·2Na ; 2.5 g/L Trypsin

DMSO（100%） Dissolvent

1000 mg/L D-Glucose ; 2200 mg/L NaHCO₃ ; 10 mg/L Phenol red ; 200 mg/L CaCl₂ (anhydrous) ; 200 mg/L MgSO₄·7H₂O

Ultrapure water Dissolvent

5 mM Hypoxanthine ; 0.02 mM Aminopterin ; 0.8 mM Thymidine ; 0.85% NaCl Dissolvent

Ultrapure water Dissolvent