PB180519
$ 25.00
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2026) IF: 3.3
DOI: 10.1007/s12010-026-05631-y
Product Cited: 5 mg/mL MTT solution
Journal: JOURNAL OF PESTICIDE SCIENCE (2026) IF: 1.5
Product Cited: 5 mg/mL MTT solution, General Freezing Medium
Journal: Advanced Materials Technologies (2025) IF: 6.2
Product Cited: 5 mg/mL MTT solution
Journal: Journal of Materials Chemistry B (2025) IF: 5.7
DOI: 10.1039/D5TB01829C
Product Cited: 5 mg/mL MTT solution, MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum
Journal: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2025) IF: 4.7
DOI: 10.1016/j.ejps.2025.107176
Product Cited: 5 mg/mL MTT solution
