auxiliary reagents

200 mM L-Alanyl-L-Glutamine ; 1 mM-4 mM Recommended working concentration ; 0.85% NaCl Dissolvent

100 mM Sodium pyruvate ; Ultrapure water Dissolvent ; 1 mM Recommended working concentration

238.3 g/L HEPES ; Purified Water Dissolvent

150,000-300,000 Molecular weight ; 10 mM PBS Dissolvent

Purified Water Dissolvent

10 mM PBS Dissolvent ; 0.2 g/L EDTA·2Na

10 mM PBS Dissolvent ; 2.5 g/L Trypsin

5% DMSO ; 40% Nutrients ; 55% DMEM/F12 ; ≥ 90% Cell viability

EBSS Dissolvent

2000 mg/L D-Glucose ; 1260 mg/L NaHCO₃ ; 30.8 mg/L CaCl₂ (anhydrous) ; 232 mg/L MgSO₄·7H₂O

0.9% Normal Saline Dissolvent

Purified Water Dissolvent

9000 mg/L NaCl ; Ultrapure water Dissolvent

1000 mg/L D-Glucose ; 350 mg/L NaHCO₃ ; 10 mg/L Phenol red ; 140 mg/L CaCl₂ (anhydrous) ; 100 mg/L MgCl₂·6H₂O ; 100 mg/L MgSO₄·7H₂O

DMSO（100%） Dissolvent

1000 mg/L D-Glucose ; 2200 mg/L NaHCO₃