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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: Scientific Reports (2022) IF: 5
DOI: 10.1038/s41598-022-19523-8
Product Cited: 5 mg/mL MTT solution, Dimethyl Sulfoxide
Journal: Bioengineered (2022) IF: 4.9
DOI: 10.1080/21655979.2022.2031676
Product Cited: 5 mg/mL MTT solution
Journal: Bioengineered (2022) IF: 4.9
DOI: 10.1080/21655979.2022.2047398
Product Cited: 5 mg/mL MTT solution
Journal: Connective Tissue Research (2022) IF: 2.9
DOI: 10.1080/03008207.2021.2025224
Product Cited: 5 mg/mL MTT solution
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
DOI: 10.1016/j.intimp.2021.108283
Product Cited: Fetal Bovine Serum, Hacat Cells Line, 5 mg/mL MTT solution, MEM, with NEAA Medium
