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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: Translational Oncology (2023) IF: 5
DOI: 10.1016/j.tranon.2023.101839
Product Cited: 5 mg/mL MTT solution, MEM Medium
Journal: Journal of Functional Biomaterials (2023) IF: 4.8
DOI: 10.3390/jfb14020077
Product Cited: 5 mg/mL MTT solution
Journal: DIAMOND AND RELATED MATERIALS (2023) IF: 4.1
DOI: 10.1016/j.diamond.2023.109702
Product Cited: 5 mg/mL MTT solution, Fetal Bovine Serum, DMEM(High glucose)
Journal: Heliyon (2023) IF: 4
DOI: 10.1016/j.heliyon.2023.e20184
Product Cited: Fetal Bovine Serum, MEM Medium, 5 mg/mL MTT solution
Journal: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES (2023) IF: 3.3
DOI: 10.1002/kjm2.12661
Product Cited: 5 mg/mL MTT solution
