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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: JOURNAL OF NANOBIOTECHNOLOGY (2023) IF: 10.2
DOI: 10.1186/s12951-023-01907-1
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, Phosphate Buffer (PBS), 5 mg/mL MTT solution, RPMI-1640
Journal: Journal of Pharmaceutical Analysis (2023) IF: 8.8
DOI: 10.1016/j.jpha.2023.12.013
Product Cited: DMEM (High glucose) Medium, 5 mg/mL MTT solution, Phosphate Buffer (PBS, 1 ×), 0.25% Trypsin Solution, Fetal Bovine Serum
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023) IF: 8.2
DOI: 10.1016/j.ijbiomac.2023.127312
Product Cited: 5 mg/mL MTT solution
Journal: JACS Au (2023) IF: 8
Product Cited: DMEM/F12, 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100×, EGF/Urogastrone/URG, Human, Recombinant, Donor Equine Serum, 5 mg/mL MTT solution
Journal: BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.5
DOI: 10.1016/j.biopha.2023.115488
Product Cited: 5 mg/mL MTT solution
