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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: PROGRESS IN ORGANIC COATINGS (2024) IF: 6.5
DOI: 10.1016/j.porgcoat.2024.108441
Product Cited: 5 mg/mL MTT solution, NCTC Clone 929 Cell Complete Medium
Journal: TALANTA (2024) IF: 5.6
DOI: 10.1016/j.talanta.2024.126483
Product Cited: 5 mg/mL MTT solution
Journal: JOURNAL OF ETHNOPHARMACOLOGY (2024) IF: 4.8
DOI: 10.1016/j.jep.2024.118934
Product Cited: 5 mg/mL MTT solution, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, McCoy's 5A Medium
Journal: Coatings (2024) IF: 3.4
Product Cited: 5 mg/mL MTT solution, Dimethyl Sulfoxide
Journal: Frontiers in Bioscience-Landmark (2024) IF: 3.3
Product Cited: Rat Bone Marrow Mesenchymal Stem Cells, 5 mg/mL MTT solution
