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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Journal: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2025) IF: 4.3
DOI: 10.1016/j.saa.2025.125743
Product Cited: 5 mg/mL MTT solution
Journal: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2025) IF: 3.6
DOI: 10.1080/09205063.2025.2501101
Product Cited: 5 mg/mL MTT solution
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2025) IF: 3.3
DOI: 10.1016/j.taap.2025.117226
Product Cited: Penicillin-Streptomycin Solution, 100 ×, 5 mg/mL MTT solution
Journal: Journal of Hard Tissue Biology (2025) IF: 0.4
DOI: 10.2485/jhtb.34.171
Product Cited: 5 mg/mL MTT solution
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 8.2
DOI: 10.1016/j.ijbiomac.2024.130016
Product Cited: DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, 0.05% Trypsin Solution, dissolved in PBS, 5 mg/mL MTT solution, Phosphate Buffer (PBS)
