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ManualPB180519
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Product Introduction
| Detection Principle | MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490 nm or 570 nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells. |
| Storage | This product can be stored at -5~-20℃ for 18 months with shading light. |
| Expiration Date | 18 months |
| Shipping | Ice bag |
Publications
Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
Journal: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES (2020) IF: 1.238
Product Cited: 5 mg/mL MTT solution
Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells
Journal: Molecules (2018) IF: 4.6
DOI: 10.3390/molecules23082012
Product Cited: DMEM(High glucose), Phosphate Buffer (PBS), 5 mg/mL MTT solution
