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ManualPM150312B
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.
4. It is not suitable for long time storage at room temperature.
5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12 Complete Medium (10) complies with ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 125mL×4 |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.4-7.8 |
| Components | DMEM/F12 [PM150312]+10% Nutrient+1% Supplement |
| Green Features | Sustainable packaging |
| Valid Period | 6 months |
| Storage Condition | This product can be stored at 2-8℃ for 6 months with shading light. |
| Shipping Condition | Ice bag |
Related Products
Publications
Journal: COMPUTATIONAL BIOLOGY AND CHEMISTRY (2026) IF: 3.1
DOI: 10.1016/j.compbiolchem.2026.108985
Product Cited: Penicillin-Streptomycin Solution, 100 ×, DMEM (High glucose) Medium, DMEM/F12 Complete Medium (10), Fetal Bovine Serum, 0.25% Trypsin Solution, with EDTA, phenol red, dissolved in PBS
Journal: FITOTERAPIA (2026) IF: 2.6
DOI: 10.1016/j.fitote.2026.107119
Product Cited: Penicillin-Streptomycin Solution, 100 ×, DMEM (High glucose) Medium, MEM, with NEAA, Fetal Bovine Serum, DMEM/F12 Complete Medium (10)
Journal: Signal Transduction and Targeted Therapy (2025) IF: 40.8
DOI: 10.1038/s41392-025-02245-4
Product Cited: HL-1 Cells Line, Tyrode's Solution, without calcium, Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Complete Medium (10), MEM, with NEAA Medium
Journal: ACS Applied Materials & Interfaces (2025) IF: 8.2
Product Cited: DMEM (High glucose) Complete Medium (10), DMEM/F12 Complete Medium (10), Human Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation Medium
Journal: International Journal of Nanomedicine (2025) IF: 6.5
DOI: 10.2147/IJN.S516926
Product Cited: LoVo Cell Complete Medium, ARPE-19 Cell Complete Medium, Penicillin-Streptomycin Solution, 100 ×, SW480 [SW-480] Cell Complete Medium, DMEM/F12 Complete Medium (10), SW620 Cell Complete Medium, KGN Cell Complete Medium, Caco-2 Cell Complete Medium, Fetal Bovine Serum, RKO Cell Complete Medium, DMEM (High glucose) Medium, 200 mM L-Alanyl-L-Glutamine Solution, 100 ×
FAQs
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Q:1 Is complete culture medium a combination of basal medium and additives? Does it need to be prepared before use?
AnswerThis complete culture medium is ready for use. It consists of a basal medium supplemented with various growth factors and antibiotics, eliminating the need for extra serum or any other additional additives.
